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PHUSA Biochem Ltd primer3plus software
Primer3plus Software, supplied by PHUSA Biochem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer3plus+software/primer3plus+software/pmc11695824-64-6-13
Average 90 stars, based on 1 article reviews
primer3plus software - by Bioz Stars, 2026-09
90/100 stars

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Software:

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients
Article Snippet: .. Compatible primers were designed by Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Article Title: Combination of Hotspot Mutations With Methylation and Fragmentomic Profiles to Enhance Multi‐Cancer Early Detection
Article Snippet: Hotspot mutations were selected based on the following criteria: [ ] reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, [ ] reported actionable mutations, and [ ] mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver, and lung cancer). .. Compatible primer pairs were designed using Primer3Plus software [ ] and synthesized by PhuSa Biochem (Ho Chi Minh City, Vietnam). ..

Article Title: Combination of hotspot mutations with methylation and fragmentomic profiles to enhance Multi-Cancer Early Detection
Article Snippet: Hotspot mutations were selected based on the following criteria: (1) reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, (2) reported actionable mutations, and (3) mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver and lung cancer). .. Compatible primer pairs were designed using Primer3Plus software ( ) and synthesized by PhuSa Biochem (Ho Chi Minh City, Vietnam). ..

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients.
Article Snippet: .. Compatible primers were designed by Frontiers in Oncology 04 Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Synthesized:

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients
Article Snippet: .. Compatible primers were designed by Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Article Title: Combination of Hotspot Mutations With Methylation and Fragmentomic Profiles to Enhance Multi‐Cancer Early Detection
Article Snippet: Hotspot mutations were selected based on the following criteria: [ ] reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, [ ] reported actionable mutations, and [ ] mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver, and lung cancer). .. Compatible primer pairs were designed using Primer3Plus software [ ] and synthesized by PhuSa Biochem (Ho Chi Minh City, Vietnam). ..

Article Title: Combination of hotspot mutations with methylation and fragmentomic profiles to enhance Multi-Cancer Early Detection
Article Snippet: Hotspot mutations were selected based on the following criteria: (1) reported hotspot mutations in the COSMIC database that are recurrent in the five cancer types, (2) reported actionable mutations, and (3) mutations found in tumor tissue from the Vietnamese cancer patients according to five types of cancers (breast, colorectal, gastric, liver and lung cancer). .. Compatible primer pairs were designed using Primer3Plus software ( ) and synthesized by PhuSa Biochem (Ho Chi Minh City, Vietnam). ..

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients.
Article Snippet: .. Compatible primers were designed by Frontiers in Oncology 04 Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Mutagenesis:

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients
Article Snippet: .. Compatible primers were designed by Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients.
Article Snippet: .. Compatible primers were designed by Frontiers in Oncology 04 Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Amplification:

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients
Article Snippet: .. Compatible primers were designed by Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients.
Article Snippet: .. Compatible primers were designed by Frontiers in Oncology 04 Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Polymerase Chain Reaction:

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients
Article Snippet: .. Compatible primers were designed by Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.

Article Title: Tumor genomic profiling and personalized tracking of circulating tumor DNA in Vietnamese colorectal cancer patients.
Article Snippet: .. Compatible primers were designed by Frontiers in Oncology 04 Primer3Plus software and synthesized by PhuSa Biochem, Vietnam. cfDNA fragments carrying the selected mutation sites were amplified in a PCR reaction containing designed primer pairs and enzyme KAPA HiFi DNA Polymerase (Roche, USA). .. Amplified cfDNA fragments were indexed and sequenced on the NextSeq 2000 system (Illumina, USA) with an average depth of 100,000X per amplicon.



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